Mayank * *******
Research Associate 2
Raleigh, NC 27606, USA | (606) 545 8066
[email protected] | www.linkedin.com/in/mskapadia1
· Extensive DNA/RNA/Protein characterization experience with expertise in technique like ELISA, IHC, Gel Electrophoresis, SDS-PAGE, Western Blotting, PCR (RT/q/dd-PCR), Qubit, and NanoDrop.
· Experienced in primer designing, gene expression and array, gene knockdown, UV/VIS spectroscopy, HeLa and MDM cell and microbial culture and their maintenance, gene silencing, ELISA development, media/buffer preparation, chromatography, Fluorescence microscopy, FRET assay, immunohistochemistry (IHC), and aseptic techniques.
· Proficient in quantification of nucleic acids.
· Excellent pipetting, documentation, troubleshooting, and multitasking skills.
· Proficient in Microsoft Office tools and imaging tools like Image Studio, Adobe Photoshop, Corel Draw.
Ø MS in Biology, Western Kentucky University, Bowling Green, KY, US; August 2016.
Ø MS in Biotechnology, Vel’s College of Science, Chennai, India; April 2009.
Ø BS in Biotechnology, Microbiology, Chemistry, M.S. Ramaiah College of Arts, Science & Commerce, Bangalore, India; May 2007.
Ø Adrenas Therapeutics, Inc., Raleigh, NC, US December 2018 – till date
Research Associate II
· Performing and validating PCR-based assays to support formal gene therapy development activities for a rare genetic disease.
· Contributing in the experiment planning, analysis and interpretation of data, and technical troubleshooting.
· Performing cell culture and transduction/transfection of lead gene therapy candidate to confirm the target protein production using western blotting, ELISA, and FRET assay.
· Assisting in the development of new technical capacities, creation of SOPs, and archiving of data.
· Testing materials coming from collaborators and vector production facilities, assessment of new lead candidates coming from R&D, establishing formal potency assays for qualification internally or externally at service providers
Ø University of Texas Medical Branch, Galveston, TX, US November 2016 – November 2018
Research Associate I
· Executed AIDS clinical protocol for collection and maintenance of the “Texas Repository of AIDS Neuropathogenesis Research (TRANR)” and CHARTER samples.
· Archived collected deceased human organs along with blood, CSF, urine, and biopsy samples from HIV-positive and negative human subjects.
· Successfully extracted DNA and RNA from archived organs of the HIV-positive patients and performing qPCR and RT-PCR to map the latent virus reservoir size in the body.
· Performed RT and qPCR on the various organs of hu-NSG mouse infected with TB and HIV.
· Successfully performed expression of 320 genes on the spleen, brain, and PBMC samples of the HIV-positive patients to find a potential candidate for targeting the latent virus pool.
· Performed ELISA, IHC, and western blotting to confirm the presence of targeted protein.
· Cultured MDM and T-cells from HIV patients’ blood to test the hypothesis of the latent virus survival mechanism.
· Complied with the research standards in the designing, methodology, and documentation of experiments.
· Conducted necessary literature searches to establish new protocols and validating to support latent virus reservoir studies.
· Assisted in grant preparation, regulatory compliance, lab inspections, and quality assurance of lab instruments.
Ø Western Kentucky University, KY, US May 2016 – July 2016
Graduate Student Worker, Biotech Center
· Assisted university students with the proper use of instruments like pipettes, pH meter, UV/Vis spectroscopy, microscope, ELISA, Laminar air flow, centrifuge, sonicator, NanoDrop, and others.
· Documented in and out supplies of the biotech center.
· Cleaned and maintained biotech center equipment, classrooms, and restocking supplies.
· Assisted in multiple projects assigned to the Biotech center.
· Performed isolation, quantification, amplification, and sequencing of DNA from small insects.
Graduate Research Associate, WKU January 2014 – August 2016
· Maintained lab and equipment as per the University lab safety norms.
· Successfully designed and executed protocols for isolation of cross-linked proteins from Drosophila.
· Performed protein purification and characterization studies using techniques like SDS-PAGE, western blotting, co-immunoprecipitation, ELISA, and Mass spectrometry.
· Performed gene silencing and overexpression of target genes in Drosophila using Gal4/UAS system.
· Dissected the Drosophila larvae to analyze the tissues using fluorescence microscopy.
· Collected, organized, and studied necessary scientific literature and established new protocols which were validated and tested for the protein characterization.
· Trained and assisted three undergraduates in their respective molecular biology projects.
Ø MS in Biology, Western Kentucky University, US 2014–2016
Identification of Collagen IV Associated Proteins in Drosophila Using Genetics and Mass Spectrometry
· Isolated cross-linked proteins using formaldehyde, SDS-PAGE, western blotting, ELISA, and co-immunoprecipitation techniques.
· Successfully developed, executed, and standardized a novel protocol for the isolation of basement membrane proteins from Drosophila.
· Performed PCR, RNA knockdown, RT-PCR, primer designing, and over-expression of a single candidate obtained from mass spectrometry analysis to understand the interaction pattern.
Ø MS in Biotechnology, University of Madras, India 2008–2009
Antimicrobial and Anticancer Activity of Nanoparticle Enhanced Compounds of A. bracteolate Lam.
· Isolated active compound aristolochic acid, a cancer causing agent, of Aristolochia bracteolate using soxlet apparatus and thin layer chromatography.
· Conjugated active compound with silver nanoparticles (potential drug) and performed UV-Vis spectroscopy to determine the complex formation.
· Prepared appropriate culture mediums to maintain cell and microbial culture.
· Performed cytotoxicity and cell viability tests on conjugate-treated HEp2 cancer & Vero cell lines for the drug-cell interaction studies.
· Carried out zone of inhibition test on various disease-causing bacteria.
· Techniques: Western Blotting, SDS-PAGE, Gene Expression, Gene analysis, Protein Assay,
RT-PCR, PCR, Co-immunoprecipitation, DNA/RNA/Protein Isolation, Gene Knockdown/Silencing, ELISA, TR-FRET, Tissue Dissection, Column Chromatography, Gel Electrophoresis, mammalian cell culture and assays.
· Software: Microsoft Word, Excel, PowerPoint, Image Studio, Adobe Photoshop, Corel Draw.
MS Biology at Western Kentucky University